Identification Of Streptococcus Pneumonia

Introduction to Strep. Pnemonia

Kingdom: Bacteria
Phylum: Firmicutes
Class: Diplococci
Order: Lactobacillales
Family: Streptococcaceae
Species : Streptoccoccus Pnemonia

General Characteristic

-Gram Positive Bacteria
-Lancet-Shaped Cocci
-Diploccoci in short chain

Morphology On Blood Agar

-Alpha Hemolysis
-Mucoid Colony
-Irregular Shaped Colony

Disease cause by streptococcus pnemonia

– pneumonia
– Sinusitis
– Otitis media
– Bacteremia
– Meningitis

Spread of infection strep pneumonia

  • The streptococcus pneumoniae become dangerous when the body’s immune system is weak.
  • The bacteria are spread through coughing. Coughing releases pneumococcus to more sensitive areas in the body, or the bacteria can be breathed in from others who cough it up.
  • The spread of streptococcus pneumoniae is most commonly found in communities where people gather, such as schools, workplaces and fitness centers. Hospitals also are high-risk locations for spreading the bacteria.

Symptoms of infection

  • Fever
  • Coughing
  • Sweating and chills
  • Headache
  • Chest pain that changes with each breath
  • Muscle Pain
  • Fatigue

Identification of strep pneumonia

1. Optochin Test

-Strep. Pneumonia form a 16-mm zone of inhibition around a 5 mg optochin disc
-Other alpha hemolysis streptococci resistant to optochin for example streptococcus viridans.

2. Bile Solubility

-The purpose of the bile solubility test is to aid in the differentiation of S. pneumoniae from all other alpha-hemolytic streptococci. Sodium deoxycholate (2%) acts on the cell wall of pneumococci resulting in lysis.
-Bacteria will lyse and the area become clear
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